ABSTRACT
Objective: We conducted a study to understand the genetic effect of ERCC1 and RRM1 on the chemotherapy response and clinical outcome of Non-Small Cell Lung Cancer [NSCLC]
Methods: The relative cDNA quantification for ERCC1 and RRM1 was conducted using a fluorescence-based real-time detection method among 294 NSCLC patients
Results: Compared with the internal reference gene beta-action, the median levels of ERCC1 and RRM1 expression were 2.43x10-2 and 0.11x10-2, respectively. Our study showed response to platinum-containing regimen chemotherapy was high in those with high ERCC1 expression, and the OR [95% CI] were 1.73[1.06-2.81]. An apparently high response to chemotherapy was decreased when patients were carrying both high expression of ERCC1 and RRM1, with OR [95% CI] of 2.57[1.21-4.90]. Patients with high expression of ERCC1 were associated with a longer OS by multivariate Cox proportional hazards model. Moreover, those carrying both high levels of ERCC1 and RRM1 were seem to have a longer OS when compared with those with low expression [HR=0.31, 95% CI=0.13-0.62 for OS]
Conclusion: This observation could be used in personalized chemotherapy, increase the response rate and prolonged survival time, and could encourage to explore the predictive value of other genes